|
KMID : 0369820120420010021
|
|
Jorunal of Korean Pharmaceutical Sciences
2012 Volume.42 No. 1 p.21 ~ p.31
|
|
|
Simultaneous analysis of bioactive metabolites from Ziziphus jujuba by HPLC-DAD-ELSD-MS/MS
|
|
Liao Li Juan
Won Tae-Hyung
Kang Sam-Sik
Shin Jong-Heon
|
|
|
Abstract
|
|
|
|
A high-performance liquid chromatography (HPLC) with diode array detector (DAD), evaporative light scattering detector (ELSD) and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of nine representative metabolites of the alkaloid, flavonoid and triterpenoid classes from Ziziphus jujuba var. spinosa. The optimal chromatographic conditions were obtained on an ODS column and the mobile phase was composed of water and methanol with 0.1% formic acid using a gradient elution system. Using the developed methods, all of the validation parameters were successfully obtained. In addition, effectiveness of diverse extraction methods was compared to each other for the development of standard analytic method. The verified method was successfully applied to the quantitative determination of representative metabolites in commercial samples of Z. jujuba and Z. mauritiana from different markets in Korea, China and Myanmar. The analytical results showed that the contents of the nine analytes vary significantly with sources and species, thus demonstrating its potential for the detection of this plant.
|
|
|
KEYWORD
|
|
|
HPLC-DAD-ELSD-MS/MS, Ziziphus jujuba var. spinosa, Ziziphus mauritiana, Analytical method, Bioactive constituents
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|